pET23a-His-hRanGAP1tail

53139 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag
Depositor
Frauke Melchior
Article
Flotho et al Methods Mol Biol. 2012;832:93-110. doi:
Insert
RanGAP1 (RANGAP1 Human)
Tags
His
Mutation
C-terminal fragment (amino acids 398-587)
Promoter
T7
Available Since
May 30, 2014
Availability
Available

Sequence Information

Length
876 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 28..46 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 634..652 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 718..766 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG