pET23a-mUbc9

53137 Mus musculus Bacterial Expression

Plasmid Information

Purpose
E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)
Depositor
Frauke Melchior
Article
Pichler et al Cell. 2002 Jan 11;108(1):109-20.
Insert
Ubc9 (Ube2i Mouse)
Tags
untagged
Mutation
protein sequence is identical to human
Promoter
T7
Available Since
Sept. 17, 2014
Availability
Available

Sequence Information

Length
887 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 554..572 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 638..686 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG