pET11d-hUba2

53136 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

E.coli expression of full length untagged human Uba2 (SAE2)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

UBA2 (UBA2 Human)

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Available

Sequence Information

Length

850 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	42..61	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	61..86	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC