pET28a-His-hAos1

53135 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag
Depositor
Frauke Melchior
Article
Pichler et al Cell. 2002 Jan 11;108(1):109-20.
Insert
Aos1 (SAE1 Human)
Tags
His
Promoter
T7
Available Since
Sept. 17, 2014
Availability
Available

Sequence Information

Length
830 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 149..167 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 176..194 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 50..69 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 69..94 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC