pHL 1278

53018 Synthetic Bacterial Expression

Plasmid Information

Purpose
AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1
Depositor
Han Lim
Article
Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
Tags
RBS(st7)
Promoter
pLlacO-1 and pLtetO-1
Available Since
May 14, 2014
Availability
Available

Sequence Information

Length
783 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

protein_bind 1
Label Gene Product Position Strand
lac operator — — 36..53 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 107..194 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT