pHL 1277

53017 Synthetic Bacterial Expression

Plasmid Information

Purpose
AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1
Depositor
Han Lim
Article
Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
Tags
RBS(st7)
Promoter
pLlacO-1 and pLtetO-1
Available Since
May 14, 2014
Availability
Available

Sequence Information

Length
729 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 179..284 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 1
Label Gene Product Position Strand
tet operator tetO — 54..73 Fwd
DNA Sequence
TCTCTATCACTGATAGGGA