pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER

52689 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Same as 41022, with additional linker
Depositor
Michael Nick Boddy
Insert
tetO7-Pcyc1-3xFLAG+LINKER
Available Since
April 23, 2014
Availability
Available

Sequence Information

Length
822 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
3xFLAG — three tandem FLAG(R) epitope tags, foll… 29..101 Rev
DNA Sequence
GACTACAAGGACGACGATGACAAGGATTACAAAGATGACGACGATAAGGACTATAAGGACGATGATGATAAA
Protein Sequence
DYKDDDDKDYKDDDDKDYKDDDDK
protein_bind 1
Label Gene Product Position Strand
tetracycline response element — — 320..591 Fwd
DNA Sequence
TCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGA