pGEX4T2-Snf1-cat

52683 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
expression of the Snf1 kinase domain in bacteria
Depositor
Benjamin Turk
Article
Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.
Insert
SNF1 (SNF1 Budding Yeast)
Tags
GST
Mutation
encodes kinase domain only, aa 41-315
Promoter
tac
Available Since
April 14, 2014
Availability
Available

Sequence Information

Length
910 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 6..24 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS