pGEX4T2-Ste7-EE

52681 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
expression of constitutively active Ste7 kinase in bacteria
Depositor
Benjamin Turk
Article
Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi
Insert
Ste7 (STE7 Budding Yeast)
Tags
GST and Myc
Mutation
S359E/T363E constitutively active
Promoter
tac
Available Since
April 30, 2014
Availability
Available

Sequence Information

Length
797 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 0..18 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS