His Puc (aa 1-393)

52295 Drosophila melanogaster Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of His tagged Drosophila Puc fragment
Depositor
Brenda Schulman
Article
Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
Insert
Puc (puc Fly)
Tags
His
Promoter
T7
Available Since
Aug. 24, 2018
Availability
Available

Sequence Information

Length
863 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 207..225 Fwd
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 108..127 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 127..152 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC