pGEX-hXRCC1-K176R

52275 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of human XRCC1-K176R with N-terminal GST-tag
Depositor
Primo Schaer
Article
Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:
Insert
XRCC1 (XRCC1 Human)
Tags
GST
Mutation
K176R, SUMO acceptor site mutant
Promoter
tac
Available Since
July 10, 2014
Availability
Available

Sequence Information

Length
930 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 191..719 Rev
DNA Sequence
ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTGGAATTAGCTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAA
Protein Sequence
MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVRQPSEAVVWLCRS
promoter 2
Label Gene Product Position Strand
tac promoter — — 61..90 Rev
DNA Sequence
TTGACAATTAATCATCGGCTCGTATAATG
lac promoter — — 762..793 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 37..54 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
lac operator — — 738..755 Fwd
DNA Sequence
TTGTTATCCGCTCACAA