| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 213..231 | Fwd | |
|
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
|
|||||
| SUMO2 | — | human small ubiquitin-related modifier 2 | 252..531 | Fwd | |
|
DNA Sequence
ATGGCCGACGAAAAGCCCAAGGAAGGAGTCAAGACTGAGAACAACGATCATATTAATTTGAAGGTGGCGGGGCAGGATGGTTCTGTGGTGCAGTTTAAGATTAAGAGGCATACACCACTTAGTAAACTAATGAAAGCCTATTGTGAACGACAGGGATTGTCAATGAGGCAGATCAGATTCCGATTTGACGGGCAACCAATCAATGAAACAGACACACCTGCACAGTTGGAAATGGAGGATGAAGATACAATTGATGTGTTCCAACAGCAGACGGGAGGT
Protein Sequence
MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 114..133 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 640..659 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 133..158 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| lac operator | — | — | 659..684 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||