pSA1

52261 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1
Depositor
Primo Schaer
Article
Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:
Tags
6His-tag
Mutation
C-terminal gly-gly
Promoter
T7 and rbs
Available Since
July 10, 2014
Availability
Available

Sequence Information

Length
839 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 208..226 Fwd
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 109..128 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T7 promoter — — 657..676 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 128..153 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
lac operator — — 676..701 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC