pSUMO3

52260 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2
Depositor
Primo Schaer
Article
Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:
Tags
6His-tag
Mutation
C-terminal gly-gly
Promoter
T7 and rbs
Available Since
July 10, 2014
Availability
Available

Sequence Information

Length
845 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 209..227 Fwd
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
SUMO3 human small ubiquitin-related modifier 3 248..524 Fwd
DNA Sequence
ATGTCCGAGGAGAAGCCCAAGGAGGGTGTGAAGACAGAGAATGACCACATCAACCTGAAGGTGGCAGGGCAGGACGGCTCCGTGGTGCAGTTCAAGATCAAGAGGCACACGCCGCTGAGCAAGCTGATGAAGGCCTACTGCGAGAGGCAGGGCTTGTCAATGAGGCAGATCAGATTCAGGTTCGACGGGCAGCCAATCAATGAAACTGACACTCCAGCACAGCTGGAGATGGAGGACGAGGACACCATCGACGTGTTCCAGCAGCAGACGGGAGGT
Protein Sequence
MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG
promoter 2
Label Gene Product Position Strand
T7 promoter 110..129 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T7 promoter 669..688 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator 129..154 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
lac operator 688..713 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC