pSUMO2

52259 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2
Depositor
Primo Schaer
Article
Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:
Tags
6His-tag
Mutation
C-terminal gly-gly
Promoter
T7 and rbs
Available Since
July 10, 2014
Availability
Available

Sequence Information

Length
878 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 217..235 Fwd
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
SUMO2 human small ubiquitin-related modifier 2 256..535 Fwd
DNA Sequence
ATGGCCGACGAAAAGCCCAAGGAAGGAGTCAAGACTGAGAACAACGATCATATTAATTTGAAGGTGGCGGGGCAGGATGGTTCTGTGGTGCAGTTTAAGATTAAGAGGCATACACCACTTAGTAAACTAATGAAAGCCTATTGTGAACGACAGGGATTGTCAATGAGGCAGATCAGATTCCGATTTGACGGGCAACCAATCAATGAAACAGACACACCTGCACAGTTGGAAATGGAGGATGAAGATACAATTGATGTGTTCCAACAGCAGACGGGAGGT
Protein Sequence
MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG
promoter 2
Label Gene Product Position Strand
T7 promoter 118..137 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T7 promoter 673..692 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
lac operator 137..162 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
lac operator 692..717 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC