pMZ285

52226 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Expression of sgRNA precursor targeted against ade6M210
Depositor
Mikel Zaratiegui
Article
Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
Insert
rrk1-sgRNA
Promoter
rrk1
Available Since
Oct. 29, 2014
Availability
Available

Sequence Information

Length
872 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 2
Label Gene Product Position Strand
rrk1 promoter/leader 184..541 Rev
DNA Sequence
TTTGCTTATGTTGGTGGTAGTTGGCATGCGTAGACTGATGACTAGTCAGCAAGGAGCGTAGAACAGTCACACTCGTTATATATGTGCTTCCAAGAAAACTCAAGAATTTACCATTAGCAAACACTTTTTTGAAATGTTAGACATTTAAATGACGAAGGCATATAGAAGCTTTGAATAGGTGTTGTAAAGTGTTGATTTATGTGACGCTGAGGGTGCGCATGAAAGGAATGTTGGGTCACGATTATTAAACAGTTTGCTAGCTTGGACACTTGAGTATTGGAAGTTGTTGAATTCTAAAAAACTTTCAGTTGATTTGAATAGTTGCTGTTGCCAAAAAACATAACCTGTACCGAAGAA
lac promoter 625..656 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 601..618 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 670..692 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA