pMZ283

52224 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).
Depositor
Mikel Zaratiegui
Article
Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
Insert
rrk1-sgRNA
Promoter
rrk1
Available Since
Oct. 29, 2014
Availability
Available

Sequence Information

Length
889 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 2
Label Gene Product Position Strand
rrk1 promoter/leader 196..553 Rev
DNA Sequence
TTTGCTTATGTTGGTGGTAGTTGGCATGCGTAGACTGATGACTAGTCAGCAAGGAGCGTAGAACAGTCACACTCGTTATATATGTGCTTCCAAGAAAACTCAAGAATTTACCATTAGCAAACACTTTTTTGAAATGTTAGACATTTAAATGACGAAGGCATATAGAAGCTTTGAATAGGTGTTGTAAAGTGTTGATTTATGTGACGCTGAGGGTGCGCATGAAAGGAATGTTGGGTCACGATTATTAAACAGTTTGCTAGCTTGGACACTTGAGTATTGGAAGTTGTTGAATTCTAAAAAACTTTCAGTTGATTTGAATAGTTGCTGTTGCCAAAAAACATAACCTGTACCGAAGAA
lac promoter 637..668 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 613..630 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 682..704 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA