pMZ222

52223 Streptococcus pyogenes Yeast Expression

Plasmid Information

Purpose
Constitutive expression of humanized Cas9
Depositor
Mikel Zaratiegui
Article
Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
Insert
Cas9
Mutation
silent mutation in the original CspCI site
Promoter
adh1
Available Since
Oct. 29, 2014
Availability
Available

Sequence Information

Length
896 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
SV40 NLS nuclear localization signal of SV40 (si… 2..23 Rev
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV