pChlr-8SE

52172 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 284-319 (PUM1 Human)
Mutation
Changed 12N to 12S and 16Q to 16E in module 8
Available Since
April 28, 2014
Availability
Available

Sequence Information

Length
812 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 194..225 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 170..187 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 239..261 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA