pChlr-7SYR

52170 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 241-283 (PUM1 Human)
Mutation
Changed 13N to 13Y and 16E to 16R in module 7
Available Since
May 5, 2014
Availability
Available

Sequence Information

Length
941 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 279..310 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 255..272 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 324..346 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA