pChlr-7NQ

52169 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 241-283 (PUM1 Human)
Mutation
Changed 12S to 12N and 16E to 16Q in module 7
Available Since
May 5, 2014
Availability
Available

Sequence Information

Length
875 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 279..310 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 255..272 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 324..346 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA