pChlr-6SE — PlasmidDB

Plasmid Information

Purpose

Encodes module 6 with amino acids 12S-16E for recognition of nt 3G

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 205-240 (PUM1 Human)

Mutation

Changed 12N to 12S and 16Q to 16E in Module 6

Available Since

April 28, 2014

Availability

Available

Length

815 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	197..228	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	173..190	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	242..264	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA