pChlr-5SR

52162 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 169-204 (PUM1 Human)
Mutation
Changed 12C to 12S and 16Q to 16R in Module 5
Available Since
April 28, 2014
Availability
Available

Sequence Information

Length
816 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 197..228 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 173..190 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 242..264 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA