pChlr-5SE — PlasmidDB

Plasmid Information

Purpose

Encodes module 5 with amino acids 12S-16E for recognition of nt 4G

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 169-204 (PUM1 Human)

Mutation

Changed 12C to 12S and 16Q to 16E in Module 5

Available Since

April 28, 2014

Availability

Available

Length

819 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	200..231	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	176..193	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	245..267	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA