pChlr-4SYR — PlasmidDB

Plasmid Information

Purpose

Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 133-168 (PUM1 Human)

Mutation

Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…

Available Since

April 28, 2014

Availability

Available

Length

822 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	204..235	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	180..197	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	249..271	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA