pChlr-4SYE

52156 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
D43951 (PUM1 Human)
Mutation
Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…
Available Since
April 28, 2014
Availability
Available

Sequence Information

Length
821 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 203..234 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 179..196 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 248..270 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA