pChlr-4CYQ — PlasmidDB

Plasmid Information

Purpose

Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 133-168 (PUM1 Human)

Mutation

Changed 12N to 12C and 13H to 13Y in Module 4

Available Since

April 28, 2014

Availability

Available

Length

767 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	204..235	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	180..197	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	249..271	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA