pChlr-4CQ

52151 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 133-168 (PUM1 Human)
Mutation
Changed 12N to 12C in Module 4
Available Since
April 28, 2014
Availability
Available

Sequence Information

Length
765 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 202..233 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 178..195 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 247..269 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA