pChlr-3NQ

52149 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 97-132 (PUM1 Human)
Mutation
Changed 12C to 12N in Module 3
Available Since
April 28, 2014
Availability
Available

Sequence Information

Length
817 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 199..230 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 175..192 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 244..266 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA