pChlr-3CQ

52147 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A
Depositor
Huimin Zhao
Article
Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
Insert
Pumilio homology domain amino acids 97-132 (PUM1 Human)
Available Since
May 5, 2014
Availability
Available

Sequence Information

Length
783 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 195..226 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 171..188 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 240..262 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA