pChlr-2NQ — PlasmidDB

Plasmid Information

Purpose

Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 61-96 (PUM1 Human)

Available Since

April 28, 2014

Availability

Available

Length

820 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	200..231	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	176..193	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	245..267	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA