pChlr-2CQ — PlasmidDB

Plasmid Information

Purpose

Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 61-96 (PUM1 Human)

Mutation

Changed 12N to 12C in Module 2

Available Since

April 28, 2014

Availability

Available

Length

914 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	186..217	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	162..179	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	231..253	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA