pChlr-1SR — PlasmidDB

Plasmid Information

Purpose

Encodes module 1 with amino acids 12S-16R for recognition of nt 8C

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 25-60 (PUM1 Human)

Mutation

Changed 16Q to 16R in Module 1

Available Since

May 5, 2014

Availability

Available

Length

847 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
lac promoter	—	—	185..216	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	161..178	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	230..252	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA