pGEM-and4 in situ probe

52105 Danio rerio Bacterial Expression

Plasmid Information

Purpose
actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol
Depositor
Didier Stainier
Article
Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:
Insert
actinodin 4 in situ probe (and4 Zebrafish)
Available Since
April 15, 2014
Availability
Available

Sequence Information

Length
1,055 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 3
Label Gene Product Position Strand
T7 promoter — — 5..24 Fwd
DNA Sequence
TAATACGACTCACTATAGG
SP6 promoter — — 880..899 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
lac promoter — — 964..995 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 940..957 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 1009..1031 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA