pGEM-and3 in situ probe

52104 Danio rerio Bacterial Expression

Plasmid Information

Purpose
actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol
Depositor
Didier Stainier
Article
Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:
Insert
actinodin 3 in situ probe (and3 Zebrafish)
Available Since
March 26, 2014
Availability
Available

Sequence Information

Length
1,069 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 3
Label Gene Product Position Strand
T7 promoter 5..24 Fwd
DNA Sequence
TAATACGACTCACTATAGG
SP6 promoter 874..893 Rev
DNA Sequence
ATTTAGGTGACACTATAGA
lac promoter 958..989 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 934..951 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 1003..1025 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA