SUMO-KTag

51723 Synthetic Bacterial Expression

Plasmid Information

Purpose
Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
Depositor
Mark Howarth
Article
Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
Insert
SUMO-KTag
Tags
His6 Tag
Mutation
none
Promoter
T7
Available Since
May 1, 2014
Availability
Available

Sequence Information

Length
959 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 56..74 Fwd
DNA Sequence
CATCACCATCATCATCAC
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 429..447 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
rep_origin 1
Label Gene Product Position Strand
f1 ori — — 597..956 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 513..561 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG