pET20b- hTPI Ile170Val

50724 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
overexpression of the human TPI1 Ile170Val allele in E.coli
Depositor
Markus Ralser
Article
Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:
Insert
Triosephosphate isomerase 1 (TPI1 Human)
Tags
6xHIS tag
Mutation
changed Isoleucine 170 to Valine
Promoter
T7
Available Since
Jan. 31, 2014
Availability
Available

Sequence Information

Length
775 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
6xHis 6xHis affinity tag 26..44 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH