pET20b- hTPI

50723 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
overexpression of human TPI1 in E.coli
Depositor
Markus Ralser
Article
Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:
Insert
Triosephosphate isomerase 1 (TPI1 Human)
Tags
6xHIS tag
Promoter
T7
Available Since
Jan. 30, 2014
Availability
Available

Sequence Information

Length
917 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 27..45 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 862..881 Rev
DNA Sequence
TAATACGACTCACTATAGG