pDBD-M

50572 Synthetic Yeast Expression

Plasmid Information

Purpose
Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
Depositor
Alexander Varshavsky
Article
Turner et al Science. 2000 Sep 22;289(5487):2117-20.
Insert
DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
Promoter
Gal1
Available Since
Feb. 14, 2014
Availability
Available

Sequence Information

Length
785 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 276..504 Rev
DNA Sequence
ATGCAGATTTTCGTCAAGACTTTGACCGGTAAAACCATAACATTGGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGTATCCCTCCAGATCAACAAAGATTGATCTTTGCCGGTAGGCAGCTAGAAGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCCACCTTACATCTTGTGCTAAGGCTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG
HA HA (human influenza hemagglutinin) epit… 531..558 Rev
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 19..267 Rev
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC