pDBD-R — PlasmidDB

Plasmid Information

Purpose

Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin

Mutation

M1R (B-gal)

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Available

Length

870 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
GAL1 promoter	S. cerevisiae GAL1	—	30..472	Fwd
DNA Sequence CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG

protein_bind 1

Label	Gene	Product	Position	Strand
UAS	—	—	30..148	Fwd
DNA Sequence CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG