pBL6

50549 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses mCherry under the PLtetO-1 promoter
Depositor
Jeffrey Tabor
Article
Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm
Insert
mCherry
Available Since
June 24, 2014
Availability
Available

Sequence Information

Length
876 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 1
Label Gene Product Position Strand
PLtetO-1 promoter 152..226 Rev
DNA Sequence
TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACATCAGCAGGACGCACTGACC
protein_bind 2
Label Gene Product Position Strand
tet operator tetO 182..201 Fwd
DNA Sequence
TCTCTATCACTGATAGGGA
tet operator tetO 207..226 Fwd
DNA Sequence
TCTCTATCACTGATAGGGA