pRS303-pGAL-HA-SrtAstrep (pKS78)

Plasmid Information

Purpose

Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Length

798 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
HA	—	HA (human influenza hemagglutinin) epit…	512..539	Fwd
DNA Sequence TACCCATACGACGTACCAGACTACGCA Protein Sequence YPYDVPDYA
thrombin site	—	thrombin recognition and cleavage site	548..566	Fwd
DNA Sequence CTGGTGCCGCGCGGATCC Protein Sequence LVPRGS

promoter 1

Label	Gene	Product	Position	Strand
GAL1 promoter	S. cerevisiae GAL1	—	32..474	Fwd
DNA Sequence CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG

protein_bind 1

Label	Gene	Product	Position	Strand
UAS	—	—	32..150	Fwd
DNA Sequence CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG