SRAcode_in_pUC57_v3(767-999)

Plasmid Information

Purpose

for transcribing SRA nucl 767-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

Available Since

Jan. 16, 2014

Availability

Available

Length

820 bp

Topology

linear

Molecule Type

genomic DNA

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	256..275	Rev
DNA Sequence TAATACGACTCACTATAGG
AmpR promoter	bla	—	558..663	Fwd
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT