SLIRP.HMG6.wt.fl

49821 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expression vector for MBP-SLIRP fusion
Depositor
Thomas Cech
Article
McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.
Insert
SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
Tags
E. coli Maltose Binding Protein
Mutation
wildtype codon-optimized for E. coli expression
Promoter
T7
Available Since
Jan. 16, 2014
Availability
Available

Sequence Information

Length
811 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 380..398 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 464..512 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG