pRKO — PlasmidDB

Plasmid Information

Purpose

loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

loxP-kanMX-loxP cassette

Promoter

none

Available Since

Dec. 5, 2013

Availability

Available

Sequence Information

Length

777 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

terminator 1

Label	Gene	Product	Position	Strand
TEF terminator	—	—	76..274	Rev
DNA Sequence ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG