CS2+MYC-Etv5:T139D

49097 Danio rerio Bacterial Expression

Plasmid Information

Purpose
expression of Etv5 T139D mutant
Depositor
Michael Tsang
Article
Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub
Insert
Etv5 T139D (etv5a Zebrafish)
Tags
MYC
Mutation
T139D
Promoter
CMV
Available Since
Nov. 22, 2013
Availability
Available

Sequence Information

Length
802 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 6
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 100..130 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 139..169 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 178..208 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 217..247 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 256..286 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 319..349 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
SP6 promoter — — 32..51 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA