pRsetB-PercevalHR — PlasmidDB

Plasmid Information

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Length

901 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
VC155	—	C-terminal fragment of mVenus for use i…	294..546	Rev
DNA Sequence GACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTTCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG Protein Sequence DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
7xHis	—	6xHis affinity tag	747..768	Rev
DNA Sequence CATCACCATCACCATCACCAT Protein Sequence HHHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	835..854	Rev
DNA Sequence TAATACGACTCACTATAGG