pTPGI_dCas9_VP64

49013 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
encodes yeast-optimized dCas9_VP64 synthetic transcription factor
Depositor
Timothy Lu
Article
Farzadfard et al ACS Synth Biol. 2013 Sep 11.
Insert
dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
Tags
SV40 NLS
Promoter
pTPGI (galactose+aTc inducible)
Available Since
Jan. 14, 2014
Availability
Available

Sequence Information

Length
10,757 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
SV40 NLS nuclear localization signal of SV40 (si… 715..736 Fwd
DNA Sequence
CCAAAGAAGAAGAGAAAAGTT
Protein Sequence
PKKKRKV
dCas9 catalytically dead mutant of the Cas9 e… 745..4846 Fwd
DNA Sequence
GATAAGAAATACTCTATTGGTTTGGCTATCGGTACAAACTCTGTTGGTTGGGCTGTTATTACTGATGAATACAAGGTTCCATCCAAGAAGTTCAAGGTTTTGGGTAACACTGATAGACACTCCATCAAAAAGAACTTGATTGGTGCCTTGTTGTTCGATTCTGGTGAAACTGCTGAAGCTACTAGATTGAAAAGAACCGCTAGAAGAAGATACACCAGAAGAAAGAACAGAATCTGCTACTTGCAAGAAATCTTCTCCAACGAAATGGCCAAGGTTGATGATTCATTCTTCCACAGATTGGAAGAATCCTTCTTGGTCGAAGAAGATAAGAAGCACGAAAGACATCCAATCTTCGGTAACATCGTTGATGAAGTTGCTTACCACGAAAAGTACCCAACTATCTACCATTTGAGAAAGAAGTTGGTTGACTCTACCGATAAGGCTGATTTGAGATTGATCTATTTGGCTTTGGCCCACATGATTAAGTTCAGAGGTCATTTCTTGATCGAAGGTGATTTGAACCCAGATAACTCCGATGTTGATAAGTTGTTCATCCAATTAGTCCAAACCTACAATCAATTATTCGAAGAAAACCCAATCAACGCCTCTGGTGTTGATGCTAAAGCTATTTTGTCTGCCAGATTGTCCAAGTCCAGAAGATTAGAAAATTTGATCGCCCAATTACCAGGTGAAAAGAAGAATGGTTTGTTCGGTAATTTGATTGCCTTGTCTTTGGGTTTGACTCCAAACTTCAAGTCCAATTTCGATTTGGCTGAAGATGCCAAGTTGCAATTATCTAAGGATACCTACGATGACGATTTGGATAACTTGTTGGCTCAAATCGGTGATCAATACGCTGATTTGTTTTTGGCTGCTAAGAACTTGTCCGATGCCATTTTGTTGTCCGATATTTTGAGAGTCAACACCGAAATTACTAAGGCTCCATTGTCTGCCTCTATGATCAAAAGATACGATGAACACCACCAAGACTTGACTTTGTTGAAGGCTTTGGTCAGACAACAATTACCTGAAAAGTACAAAGAAATTTTCTTCGATCAATCCAAGAACGGTTACGCCGGTTATATTGATGGTGGTGCTTCTCAAGAAGAATTTTACAAGTTCATCAAGCCAATCTTGGAAAAGATGGACGGTACTGAAGAATTATTGGTCAAGTTGAACAGAGAAGATTTGTTGAGAAAGCAAAGAACCTTCGACAACGGTTCTATTCCACATCAAATTCACTTGGGTGAATTGCACGCAATTTTGAGAAGACAAGAAGATTTTTATCCATTCTTGAAGGACAACAGAGAAAAGATCGAAAAGATTCTGACCTTCAGAATCCCTTACTACGTTGGTCCATTGGCTAGAGGTAATTCAAGATTTGCCTGGATGACTAGAAAGTCCGAAGAAACTATTACTCCTTGGAACTTCGAAGAAGTTGTAGATAAGGGTGCTTCTGCCCAATCCTTTATTGAAAGAATGACCAACTTCGACAAGAACTTGCCAAACGAAAAGGTTTTGCCAAAGCACTCTTTGTTGTACGAATACTTCACCGTCTACAACGAATTGACTAAGGTTAAGTACGTCACCGAAGGTATGAGAAAACCAGCTTTTTTATCCGGTGAACAAAAGAAGGCTATCGTCGATTTGTTGTTCAAGACCAACAGAAAGGTTACTGTCAAGCAATTAAAAGAAGATTACTTCAAGAAAATCGAATGCTTCGACTCCGTTGAAATTTCTGGTGTCGAAGATAGATTCAATGCCTCTTTAGGTACTTACCATGACTTGTTGAAAATCATCAAGGACAAGGATTTCTTGGACAACGAAGAAAACGAAGATATTTTGGAAGATATTGTCTTGACATTGACCTTGTTTGAAGATAGAGAAATGATTGAAGAAAGATTGAAAACCTACGCCCACTTGTTCGATGATAAGGTTATGAAGCAATTAAAGAGAAGAAGATACACTGGTTGGGGTAGATTGTCCAGAAAATTGATTAACGGTATCAGAGACAAGCAATCCGGTAAGACCATTTTGGACTTTTTGAAGTCTGATGGTTTCGCTAACAGAAACTTCATGCAATTAATCCACGACGATTCCTTGACTTTCAAAGAAGATATACAAAAGGCCCAAGTCTCTGGTCAAGGTGATTCTTTACATGAACATATCGCTAACTTGGCTGGTTCTCCAGCTATTAAGAAGGGTATTTTACAAACCGTTAAGGTCGTTGACGAATTGGTCAAAGTTATGGGTAGACATAAGCCAGAAAACATCGTTATCGAAATGGCTAGAGAAAATCAAACCACCCAAAAGGGTCAAAAGAACTCCAGAGAAAGAATGAAGAGAATCGAAGAAGGTATCAAAGAATTGGGTTCCCAAATTTTGAAAGAACACCCAGTTGAAAACACCCAATTACAAAACGAAAAGTTGTACTTGTACTACTTGCAAAACGGTAGAGATATGTACGTTGACCAAGAATTGGACATCAACAGATTGTCTGATTACGATGTTGACGCTATCGTTCCACAATCTTTTTTGAAGGATGACTCCATTGACAACAAGGTCTTGACTAGATCCGATAAGAATAGAGGTAAGTCCGATAACGTTCCATCTGAAGAAGTCGTTAAGAAAATGAAGAACTATTGGAGACAATTATTGAACGCCAAGTTGATCACCCAAAGAAAGTTTGACAATTTGACCAAGGCTGAAAGAGGTGGTTTGTCTGAATTGGATAAGGCAGGTTTCATCAAAAGACAATTAGTAGAAACCAGACAAATCACCAAGCACGTTGCTCAAATTTTGGATAGTAGAATGAACACTAAGTACGACGAAAACGACAAATTGATCAGAGAAGTTAAGGTCATTACCTTGAAGTCCAAGTTGGTTTCCGATTTCAGAAAGGACTTCCAATTCTACAAGGTCAGAGAAATCAACAACTACCATCATGCACATGATGCTTACTTGAATGCTGTTGTTGGTACTGCCTTGATTAAGAAGTATCCAAAGTTGGAATCCGAATTTGTCTACGGTGATTACAAGGTTTACGACGTTAGAAAGATGATCGCCAAGTCCGAACAAGAAATTGGTAAAGCTACTGCCAAATACTTCTTCTACTCCAATATTATGAATTTCTTTAAGACCGAAATCACTTTGGCCAACGGTGAAATTAGAAAAAGACCATTGATTGAAACTAATGGTGAAACAGGTGAAATCGTTTGGGATAAGGGTAGAGATTTTGCCACTGTTAGAAAGGTATTGTCCATGCCACAAGTAAACATCGTCAAAAAGACCGAAGTTCAAACTGGTGGTTTCTCCAAAGAATCCATTTTGCCTAAGAGAAACTCCGATAAGTTGATCGCTAGAAAAAAAGACTGGGACCCAAAAAAGTACGGTGGTTTTGATTCTCCAACTGTTGCTTACTCTGTTTTGGTTGTTGCTAAGGTCGAAAAGGGTAAGAGTAAGAAGTTGAAGTCCGTCAAAGAATTATTAGGTATCACTATCATGGAAAGATCCTCATTCGAAAAGAATCCTATCGACTTTTTGGAAGCCAAGGGTTACAAAGAAGTCAAGAAGGACTTGATCATTAAGTTGCCAAAGTACAGTTTGTTCGAATTGGAAAATGGTAGAAAGAGAATGTTGGCTTCTGCCGGTGAATTACAAAAGGGTAATGAATTGGCTTTGCCATCCAAGTACGTTAATTTCTTATACTTGGCCTCCCACTACGAAAAATTGAAAGGTTCTCCTGAAGATAACGAACAAAAGCAATTATTTGTCGAACAACACAAGCACTACTTGGACGAAATCATTGAACAAATTTCCGAATTTTCCAAAAGAGTCATTTTGGCTGACGCCAATTTGGACAAAGTTTTGTCAGCTTACAACAAGCACAGAGATAAGCCAATTAGAGAACAAGCTGAAAACATCATTCACTTGTTCACTTTGACTAACTTGGGTGCTCCAGCTGCTTTTAAGTATTTCGATACCACTATCGACAGAAAGAGATACACCTCTACCAAAGAAGTTTTGGACGCTACTTTGATCCACCAATCTATTACTGGTTTGTACGAAACTAGAATCGACTTGTCTCAATTAGGTGGTGAT
Protein Sequence
DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
VP64 tetrameric repeat of the minimal activa… 4882..5032 Fwd
DNA Sequence
GACGCTTTGGACGACTTCGACTTGGATATGCTGGGTTCTGATGCGCTAGATGACTTTGACCTCGACATGCTTGGAAGTGACGCCTTAGATGATTTTGACCTGGATATGCTTGGATCAGACGCTCTGGACGATTTCGACTTAGACATGCTT
Protein Sequence
DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDML
AmpR bla beta-lactamase 6284..7145 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
TRP1 S. cerevisiae TRP1 phosphoribosylanthranilate isomerase, r… 7817..8492 Fwd
DNA Sequence
ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG
Protein Sequence
MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRKRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK
TetR tetR tetracycline repressor TetR 9436..10060 Rev
DNA Sequence
ATGTCTAGATTAGATAAAAGTAAAGTGATTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGTAGAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGCACCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCTTTACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCAATTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAGGTTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTATTACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGGATTAGAAAAACAACTTAAATGTGAAAGTGGGTCTTAA
Protein Sequence
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS
promoter 4
Label Gene Product Position Strand
TPGI promoter 234..676 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTACTTTTTTAGCCTTATTTCTGGGGTACTGCAGCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGATATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
AmpR promoter bla 7145..7250 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
TRP1 promoter S. cerevisiae TRP1 7535..7817 Fwd
DNA Sequence
AACGACATTACTATATATATAATATAGGAAGCATTTAATAGAACAGCATCGTAATATATGTGTACTTTGCAGTTATGACGCCAGATGGCAGTAGTGGAAGATATTCTTTATTGAAAAATAGCTTGTCACCTTACGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGT
TEF1 promoter S. cerevisiae TEF1 10077..10484 Rev
DNA Sequence
CGCCACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG
protein_bind 3
Label Gene Product Position Strand
UAS 234..352 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG
tet operator tetO 559..578 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO 580..599 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
rep_origin 2
Label Gene Product Position Strand
ori 5525..6114 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
f1 ori 8594..9050 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
terminator 2
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 5093..5283 Fwd
DNA Sequence
ATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAG
ADH1 terminator S. cerevisiae ADH1 9127..9293 Rev
DNA Sequence
GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAGGTCGCTC