1132 pME-Lin28 — PlasmidDB

Plasmid Information

Purpose

full length coding sequence for zebrafish lin28 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

lin-28 homolog A (lin28ab Zebrafish)

Available Since

Oct. 28, 2013

Availability

Available

Length

920 bp

Topology

linear

Molecule Type

genomic DNA

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	836..855	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 2

Label	Gene	Product	Position	Strand
attL1	mutant version of attL	—	1..101	Fwd
DNA Sequence CCCAATATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT
attL2	mutant version of attL	—	719..819	Rev
DNA Sequence CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT