pET21 Catalytic Domain of MALT1 Catalytic Mutant

48971 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566
Depositor
Guy Salvesen
Article
Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi
Insert
MALT1 (MALT1 Human)
Tags
His
Mutation
Contains AA 329-566; Catalytic Mutant C464A
Promoter
T7
Available Since
Oct. 23, 2013
Availability
Available

Sequence Information

Length
1,131 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 38..56 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 860..879 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 835..860 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC